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Chinese Journal of Breast Disease(Electronic Edition) ›› 2012, Vol. 06 ›› Issue (04): 402-410. doi: 10.3877/cma. j. issn.1674-0807.2012.04.007

• Original Article • Previous Articles     Next Articles

Screening the sensitive protein spectrum in breast cancer tissue before and after neoadjuvant chemotherapy by SELDI-TOF MS technology

Kai YUAN1, Kun ZHANG1, Ying-lu MA1, Rong-zhan FU1,()   

  1. 1.Department of General Surgery, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan 250014, China
  • Received:2011-12-13 Online:2012-08-01 Published:2024-11-30
  • Contact: Rong-zhan FU

Abstract:

Objective

To detect protein spectrum of breast cancer tissue before and after neoadjuvant chemotherapy (NACT) using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS), in order to find the sensitive proteins to neoadjuvant chemotherapy.

Methods

We collected 60 samples of breast cancer tissues before and after NACT in 30 patients treated in Qianfoshan Hospital from March 2009 to August 2010. The breast cancer tissue sample was obtained by core needle biopsy before NACT and during radical mastectomy after NACT. SELDI-TOF MS was applied to detect the protein spectrum in breast cancer tissue and Biomarker Wizard 3.0.2 version was used for screening the differential protein spectrum. The data were analyzed by means of Shapiro-Wilk test and matching t test in SPSS17.0 software. According to the m/z of differential proteins,we retrieved in SWISS-PROT database and found the proteins in breast tissue or breast cancer tissue consistent with the differential proteins.

Results

In this study, 8 different proteins were screened in NACT group: m/z 5825.9, 8949.5, 11 053.5, 11 652.0, 17 898.9,22 250.6,26 055.5,38 546.6. They were all expressed higher after NACT (11.0±5.0 vs 8.2±4.9,10.7±4.5 vs 6.3±3.4,22.3±9.9 vs 15.3±9.9,28.0±10.2 vs 21.4±9.3, 9.5±4.6 vs 5.4±3.4,1.1±1.0 vs 0.6±0.5,2.0±0.7 vs 1.5±0.7 and 2.7±2.2 vs 1.5±1.2,P<0.05).

Conclusion

Protein spectrum which is sensitive to neoadjuvant chemotherapy in breast cancer can be screened by SELDI-TOF MS.

Key words: breast neoplasms, neoadjuvant chemotherapy, microchip analytical procedures, mass spectrometry

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